| Solution Information | help | |
| Enzyme: | Trans-activator protein BZLF1 | |
| inhibitor: | BDBM62497 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this dose response counterscreen assay is to determine whether compounds identified as active in a set of experiments entitled, experiments entitled, "Fluorescence polarization-based biochemical high throughput confirmation assay to identify inhibitors of the Epstein-Barr virus nuclear antigen 1 (EBNA-1)." (AID 2292), and inactive in a set of experiments entitled, "Counterscreen for inhibitors of EBNA-1: fluorescence polarization-based biochemical high throughput assay for inhibitors of the Epstein-Barr virus-encoded protein, ZTA, in triplicate" (AID 2328) are nonselective inhibitors due to inhibition of Zta. This assay assesses the ability of compounds to inhibit binding of the EBV-encoded protein, Zta, to its cognate DNA recognition site, ZRE1. Zta is a BZIP DNA binding domain-containing protein with homology to the cellular transcription factor c-fos (12). Zta is essential for EBV replication and is responsible for activation of the viral lytic cycle | |
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